Procedure for counting sperm cells

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A semen analysis plural: semen analysesalso called " seminogram " [1] evaluates certain characteristics of a male's semen and the sperm contained therein. It is done to help evaluate male fertilitywhether for those seeking pregnancy or verifying the success of vasectomy. Depending on the measurement method, just a few characteristics may be evaluated such as with a home kit or many characteristics may be evaluated generally by a diagnostic laboratory.

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Determining the Concentration of Sperm with a Hemocytometer. The hemocytometer is a device for counting cells or particles. As you can see below it is composed of a thick piece of glass with 2 rails on each side.

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A semen analysis, also called a sperm count, measures the quantity and quality of a man's semen and sperm. Semen is the thick, white fluid released from the penis during a man's sexual climax orgasm. This release is called ejaculation.

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Sperm is the male gametethat is, the male sex cellor the cell in males which has the capacity to fertilise an egg i. Sperm are produced in the seminiferous tubes of the testes. The pituitary gland at the base of the brain causes testosterone to be produced in the testes.

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When you see a doctor because you're having trouble getting your partner pregnant, he or she will try to determine the underlying cause. Even if your doctor thinks low sperm count is the problem, it is recommended that your partner be evaluated to rule out potential contributing factors and determine if assisted reproductive techniques may be required. This includes examination of your genitals and asking questions about any inherited conditions, chronic health problems, illnesses, injuries or surgeries that could affect fertility.

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A semen analysis measures three major factors of sperm health:. According to the American Association for Clinical Chemistry AACCthe tests should be conducted at least seven days apart and over the course of two to three months. Sperm counts can vary on a daily basis.

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A: A Haemocytometer can be used to determine the abundance of most types of cell, whether it be red cells, white cells, yeasts or spermatozoa. The rails of the haemocytometer are designed to hold a cover slip 0. This area has a grid etched into the mirrored surface. When measuring spermatozoa its important to immobilise them by killing them with water and diluting them sufficiently to be able to measure them.

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Hemacytometers were developed for counting blood cells, but can also be used to count spermatozoa. A hemacytometer has two chambers and each chamber has a microscopic grid etched on the glass surface. The chambers are overlaid with a glass coverslip that rests on pillars exactly 0.

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